Professor Edna Hardeman

Field: Biochemistry and Cell Biology

Contact Details

Phone: 9385 3760
Email: e.hardeman@unsw.edu.au

Tabs

Main

University role:

Professor

Professor Edna Hardeman is the Head of the Cellular and Genetic Medicine Unit in the School of Medical Sciences

Prof Hardeman received her doctorate from the Department of Biological Sciences, Stanford University and then took up a postdoctoral fellowship in the Department of Pharmacology, Stanford Medical School. She established her laboratory, the Muscle Development Unit at the Children’s Medical Research Institute in Sydney, and has built an international reputation defining mechanisms of muscle gene regulation, and generating mouse models for human skeletal muscle diseases and trialling therapies as part of an international consortium of the European Neuromuscular Centre. In 2009 she moved to the University of New South Wales to take up a Research Chair in Anatomy and establish the Neuromuscular and Regenerative Medicine Unit. Notable achievements include: developing the human skeletal alpha-actin promoter for skeletal muscle-specific expression in animal models of human disease and for gene therapy, the identification of a novel pulsatory, stochastic mode of gene transcription that contributed a new mechanism to the field of gene regulation, the generation of mouse models for nemaline myopathy and identification of therapies, member of collaborative team that identified tropomyosins as the gatekeepers of actin filament function, and the discovery of the gene Gtf2ird1 which is implicated in aspects of human cognition and behaviour.

Qualifications

· BA (Interdisc Arts, Hons) & BSc (Hons) The University of Texas at Austin; PhD Stanford University

Current Appointments & Positions Held

· Professor (Chair), Head of Department, Department of Anatomy, School of Medical Sciences

· Chair, National Committee for Biomedical Sciences, Australian Academy of Science

· Chair, Australian National Health & Medical Research Council (NHMRC) Animal Welfare Committee

· Hunter Cell Biology Meeting Committee

· Committee Member, Australian and New Zealand Society for Cell and Developmental Biology

· Australian National Health & Medical Research Council (NHMRC) Development Grant Expert Reviewer

· Editorial Board of BioArchitecture (link is external)

Membership in Societies

· Australian and New Zealand Society for Cell and Developmental Biology

· American Society for Cell Biology

· Australian Society for Biochemistry and Molecular Biology

· Australian Society for Medical Research

Keywords:

Cell Biology

Actin Cytoskeleton

Tropomyosin

Gene Regulation

Neuromuscular Disorders

Muscle Disease

Nemaline Myopathy

Epigenetics and Cancer

Imaging

History

Member for: 4 years 8 months

Research

Research activities:

Research Interests

The Neuromuscular and Regenerative Medicine Unit (NRMU) is engaged in a range of research activities that span basic mechanisms in cell biology to cell-based therapies to the cellular basis for aspects of human cognition and behaviour. Our disease foci are neuromuscular disorders, regenerative medicine, childhood cancer, cancer metabolic syndrome, and the neurodevelomental disorder William Beurens Syndrome. In particular, our research aims to understand muscle cytoarchitecture, muscle regeneration and develop stem cell-based therapy, to understand how radiation therapy for childhood cancers impacts the epigenome of muscle stem cells, to analyse anti-cytoarchitecture/anti-cancer drugs, and to understand how one gene, discovered by us, GTF2IRD1, can profoundly affect skeletal muscle and yet also play a role in human cognition and behaviour. Current research projects are:

· Development of anti-tropomyosin drugs, anti-cancer drugs

· Regulation of glucose uptake by tropomyosins and myosins

· Regulation of cell proliferation by the actin cytoskeleton

· The role of epigenetic modifications in longterm memory of irradiation in childhood cancer survivors

Book Chapters

Kee AJ; Hardeman EC, 2012, 'Thin filament diseases of striated muscle', in Cytoskeleton and Human Disease, pp. 123 – 140 (link is external)

Nguyen MAT; Hardeman EC, 2008, 'Mouse models for thin filament disease', in Advances in Experimental Medicine and Biology, pp. 66 - 77 (link is external)

Hardeman E; Arkell R; Brennan K; Dunwoodie S; Esser K; Gordon M; Joya J; Shalhoub C; Sutherland C, 1992, 'Skeletal muscle gene control and transgenic mice', in Kakulas B; Howell J; Roses A (ed.), Duchenne Muscular Dystrophy: Animal Models and Genetic Manipulation, edn. 1, Raven Press Ltd, New York, pp. 237 - 246

Journal articles

Pleines I; Woods J; Chappaz S; Kew V; Foad N; Ballester-Beltrán J; Aurbach K; Lincetto C; Lane RM; Schevzov G, 2017, 'Mutations in tropomyosin 4 underlie a rare form of human macrothrombocytopenia', Journal of Clinical Investigation, vol. 127, pp. 814 - 829 (link is external)

Akladios B; Mendoza-Reinoso V; Samuel MS; Hardeman EC; Khosrotehrani K; Key B; Beverdam A, 2017, 'Epidermal YAP2-5SA-ΔC Drives β-Catenin Activation to Promote Keratinocyte Proliferation in Mouse Skin In Vivo', Journal of Investigative Dermatology, vol. 137, pp. 716 - 726 (link is external)

Gunning PW; Hardeman EC, 2017, 'Tropomyosins', Current Biology, vol. 27, pp. R8 - R13 (link is external)

Appaduray MA; Masedunskas A; Bryce NS; Lucas CA; Warren SC; Timpson P; Stear JH; Gunning PW; Hardeman EC, 2016, 'Recruitment kinetics of tropomyosin tpm3.1 to actin filament bundles in the cytoskeleton is independent of actin filament kinetics', PLoS ONE, vol. 11 (link is external)

Liang H; Akladios B; Canales CP; Francis R; Hardeman EH; Beverdam A, 2016, 'CUBIC protocol visualizes protein expression at single cell resolution in whole mount skin preparations', Journal of Visualized Experiments, vol. 2016 (link is external)

Conference Papers

Tinklenberg J; Meng H; Yang L; Hardeman E; Pearsall S; Fitts R; Lawlor M, 2014, 'Treatment with ActRIIB-mFc Improves Lifespan, Behavior and Pathology in the Acta1 H40Y Murine Model of Nemaline Myopathy', in JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY, LIPPINCOTT WILLIAMS & WILKINS, Portland, OR, pp. 614 - 614, presented at 90th Annual Meeting of the American-Association-of-Neuropathologists-Inc, Portland, OR, 12 - 15 June 2014 (link is external)

Kee AJ; Lucas CA; Yang L; Szokolai RC; Martel N; Leong GM; Muscat GE; Hughes W; Cooney G; James DE, 2012, 'The actin regulatory protein Tropomyosin 5NM1 regulates glucose uptake and GLUT4 trafficking in the mouse.', in MOLECULAR BIOLOGY OF THE CELL, AMER SOC CELL BIOLOGY (link is external)

Ravenscroft G; McNamara E; Griffiths LM; Papadimitriou JM; Hardeman EC; Bakker AJ; Laing NG; Nowak KJ, 2012, 'Efficacy of cardiac actin over-expression therapy for ACTA1 disease seems mutation specific', in NEUROMUSCULAR DISORDERS, PERGAMON-ELSEVIER SCIENCE LTD, Perth, AUSTRALIA, pp. 839 - 839, presented at 17th International Congress of the World-Muscle-Society (WMS), Perth, AUSTRALIA, 09 - 13 October 2012 (link is external)

Hardeman EC; Darhed HA; Lee ASJ; Chen G; Shah J; Joya JE; Gunning PW, 2012, 'BLOOD BORNE AGENTS RESCUE REGENERATION IMPAIRED MUSCLE', in WOUND REPAIR AND REGENERATION, WILEY-BLACKWELL, pp. A59 - A59 (link is external)

Stehn JR; Haass NK; Bonello T; Desouza M; Kottyan G; Treutlein H; Zeng J; Nascimento PR; Butler T; Allanson M, 2012, 'Developing chemotherapeutics which selectively disable the actin cytoskeleton of tumor cells', in EXPERIMENTAL DERMATOLOGY, WILEY-BLACKWELL, Marburg, GERMANY, pp. e43 - e43, presented at 39th Annual Meeting of the Arbeitsgemeinschaft-Dermatologische-Forschung (ADF), Marburg, GERMANY, 01 - 03 March 2012 (link is external)

Conference Posters

Hardeman EC; Kee AJ; Lucas CA; Chagan J; Gunning PW, 2016, 'Metabolic impact of targeting the tropomyosin/actin cytoskeleton to treat cancer', in MOLECULAR BIOLOGY OF THE CELL, AMER SOC CELL BIOLOGY, San Francisco, CA, Vol. 27, presented at Annual Meeting of the American-Society-for-Cell-Biology (ASCB), San Francisco, CA, 03 - 07 December 2016 (link is external)

Suchowerska A; Chen B; Mueller J; Ittner A; Hardeman E; Ittner L; Janitz M; Gunning P; Fath T, 2013, 'Increased levels of actin filaments containing the tropomyosin Tm5NM1 regulate excitotoxicity in the mouse', in MOLECULAR BIOLOGY OF THE CELL, AMER SOC CELL BIOLOGY, Vol. 24 (link is external)

Conference Presentations

Jalilian I; Schevzov G; Fath T; Heu C; Bischof L; Vindin H; Stehn JR; Tate MK; Hardeman EC; Gunning PW, 2014, 'Determining the impact of the actin cytoskeleton on the mechanical properties of cells', in MOLECULAR BIOLOGY OF THE CELL, AMER SOC CELL BIOLOGY, Philadelphia, PA, Vol. 25, presented at ASCB/IFCB Meeting, Philadelphia, PA, 06 - 10 December 2014 (link is external)

Hardeman EC; Yang L; Kee AJ; Polishchuk E; Polishchuk R; Pleines I; Kile B; Weigert R; Gunning PW, 2014, 'A novel actin filament population defined by the tropomyosin Tm4 regulates ER-to-Golgi trafficking.', in MOLECULAR BIOLOGY OF THE CELL, AMER SOC CELL BIOLOGY, Philadelphia, PA, Vol. 25, presented at ASCB/IFCB Meeting, Philadelphia, PA, 06 - 10 December 2014 (link is external)

Brayford S; Schevzov G; Hardeman EC; Bear JE; Gunning PW, 2014, 'Tropomyosin 5a/b actin filaments collaborate with arp2/3 actin filaments to regulate cell motility.', in MOLECULAR BIOLOGY OF THE CELL, AMER SOC CELL BIOLOGY, Philadelphia, PA, Vol. 25, presented at ASCB/IFCB Meeting, Philadelphia, PA, 06 - 10 December 2014 (link is external)

Schevzov G; Wang B; Hook JW; Tonthat C; Pleines I; Kile B; Hardeman EC; Gunning PW, 2014, 'Distinct Actin Filament Networks Modulate Different Cell Signalling Pathways Involved in Cell Proliferation and Senescence.', in MOLECULAR BIOLOGY OF THE CELL, AMER SOC CELL BIOLOGY, Philadelphia, PA, Vol. 25, presented at ASCB/IFCB Meeting, Philadelphia, PA, 06 - 10 December 2014 (link is external)

Jalilian I; Schevzov G; Fath T; Heu C; Bischof L; Vindin H; Stehn JR; Tate MK; Hardeman EC; Gunning PW, 2014, 'Determining the impact of the actin cytoskeleton on the mechanical properties of cells.', in MOLECULAR BIOLOGY OF THE CELL, AMER SOC CELL BIOLOGY, Philadelphia, PA, Vol. 25, presented at ASCB/IFCB Meeting, Philadelphia, PA, 06 - 10 December 2014 (link is external)

Conference Abstracts

Eslami S; Boysen L; Amorim N; Bould S; Kee A; Lucas C; Johnston K; Hardeman E; Cohn RJ; Barres R, 2015, 'Radiation-induced metabolic complications: potential role for the microbiota', in Radiation-induced metabolic complications: potential role for the microbiota, Nutrient & Metabolite Sensing, Copenhagen, Denmark, presented at Nutrient & Metabolite Sensing, Copenhagen, Denmark, 01 - 05 November 2015

Eslami S; Boysen L; Amorim N; Bould S; Kee A; Lucas C; Johnston K; Cohn R; Hardeman E; Barres R, (eds.), 2015, 'Metabolic complications in cancer survivors: role of adipose tissue dysfunction', in Metabolic complications in cancer survivors: role of adipose tissue dysfunction, Keystone Symposia: Systems Biology of Lipid Metabolism, Breckenridge, Colorado, USA, presented at Keystone Symposia: Systems Biology of Lipid Metabolism, Breckenridge, Colorado, USA, 09 - 13 February 2015

Segueira VB; Stehn JR; Haass N; Bonello T; Guven K; Desouza M; Schevzov G; Kottyan G; McCluskey A; Bischof L, 2012, 'Improving the specificity of drugs which target the actin cytoskeleton for cancer therapy', in CANCER RESEARCH, AMER ASSOC CANCER RESEARCH, Vol. 72 (link is external)

Socio-economic Objective (SEO) tags:

Inherited Diseases (incl. Gene Therapy)

Cancer and Related Disorders

Child Health

Teaching & Supervision

I am available to supervise: