Research Interests
The Neuromuscular and Regenerative Medicine Unit (NRMU) is engaged in a range of research activities that span basic mechanisms in cell biology to cell-based therapies to the cellular basis for aspects of human cognition and behaviour. Our disease foci are neuromuscular disorders, regenerative medicine, childhood cancer, cancer metabolic syndrome, and the neurodevelomental disorder William Beurens Syndrome. In particular, our research aims to understand muscle cytoarchitecture, muscle regeneration and develop stem cell-based therapy, to understand how radiation therapy for childhood cancers impacts the epigenome of muscle stem cells, to analyse anti-cytoarchitecture/anti-cancer drugs, and to understand how one gene, discovered by us, GTF2IRD1, can profoundly affect skeletal muscle and yet also play a role in human cognition and behaviour. Current research projects are:
· Development of anti-tropomyosin drugs, anti-cancer drugs
· Regulation of glucose uptake by tropomyosins and myosins
· Regulation of cell proliferation by the actin cytoskeleton
· The role of epigenetic modifications in longterm memory of irradiation in childhood cancer survivors
Book Chapters
Kee AJ; Hardeman EC, 2012, 'Thin filament diseases of striated muscle', in Cytoskeleton and Human Disease, pp. 123 – 140 (link is external)
Nguyen MAT; Hardeman EC, 2008, 'Mouse models for thin filament disease', in Advances in Experimental Medicine and Biology, pp. 66 - 77 (link is external)
Hardeman E; Arkell R; Brennan K; Dunwoodie S; Esser K; Gordon M; Joya J; Shalhoub C; Sutherland C, 1992, 'Skeletal muscle gene control and transgenic mice', in Kakulas B; Howell J; Roses A (ed.), Duchenne Muscular Dystrophy: Animal Models and Genetic Manipulation, edn. 1, Raven Press Ltd, New York, pp. 237 - 246
Journal articles
Pleines I; Woods J; Chappaz S; Kew V; Foad N; Ballester-Beltrán J; Aurbach K; Lincetto C; Lane RM; Schevzov G, 2017, 'Mutations in tropomyosin 4 underlie a rare form of human macrothrombocytopenia', Journal of Clinical Investigation, vol. 127, pp. 814 - 829 (link is external)
Akladios B; Mendoza-Reinoso V; Samuel MS; Hardeman EC; Khosrotehrani K; Key B; Beverdam A, 2017, 'Epidermal YAP2-5SA-ΔC Drives β-Catenin Activation to Promote Keratinocyte Proliferation in Mouse Skin In Vivo', Journal of Investigative Dermatology, vol. 137, pp. 716 - 726 (link is external)
Gunning PW; Hardeman EC, 2017, 'Tropomyosins', Current Biology, vol. 27, pp. R8 - R13 (link is external)
Appaduray MA; Masedunskas A; Bryce NS; Lucas CA; Warren SC; Timpson P; Stear JH; Gunning PW; Hardeman EC, 2016, 'Recruitment kinetics of tropomyosin tpm3.1 to actin filament bundles in the cytoskeleton is independent of actin filament kinetics', PLoS ONE, vol. 11 (link is external)
Liang H; Akladios B; Canales CP; Francis R; Hardeman EH; Beverdam A, 2016, 'CUBIC protocol visualizes protein expression at single cell resolution in whole mount skin preparations', Journal of Visualized Experiments, vol. 2016 (link is external)
Conference Papers
Tinklenberg J; Meng H; Yang L; Hardeman E; Pearsall S; Fitts R; Lawlor M, 2014, 'Treatment with ActRIIB-mFc Improves Lifespan, Behavior and Pathology in the Acta1 H40Y Murine Model of Nemaline Myopathy', in JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY, LIPPINCOTT WILLIAMS & WILKINS, Portland, OR, pp. 614 - 614, presented at 90th Annual Meeting of the American-Association-of-Neuropathologists-Inc, Portland, OR, 12 - 15 June 2014 (link is external)
Kee AJ; Lucas CA; Yang L; Szokolai RC; Martel N; Leong GM; Muscat GE; Hughes W; Cooney G; James DE, 2012, 'The actin regulatory protein Tropomyosin 5NM1 regulates glucose uptake and GLUT4 trafficking in the mouse.', in MOLECULAR BIOLOGY OF THE CELL, AMER SOC CELL BIOLOGY (link is external)
Ravenscroft G; McNamara E; Griffiths LM; Papadimitriou JM; Hardeman EC; Bakker AJ; Laing NG; Nowak KJ, 2012, 'Efficacy of cardiac actin over-expression therapy for ACTA1 disease seems mutation specific', in NEUROMUSCULAR DISORDERS, PERGAMON-ELSEVIER SCIENCE LTD, Perth, AUSTRALIA, pp. 839 - 839, presented at 17th International Congress of the World-Muscle-Society (WMS), Perth, AUSTRALIA, 09 - 13 October 2012 (link is external)
Hardeman EC; Darhed HA; Lee ASJ; Chen G; Shah J; Joya JE; Gunning PW, 2012, 'BLOOD BORNE AGENTS RESCUE REGENERATION IMPAIRED MUSCLE', in WOUND REPAIR AND REGENERATION, WILEY-BLACKWELL, pp. A59 - A59 (link is external)
Stehn JR; Haass NK; Bonello T; Desouza M; Kottyan G; Treutlein H; Zeng J; Nascimento PR; Butler T; Allanson M, 2012, 'Developing chemotherapeutics which selectively disable the actin cytoskeleton of tumor cells', in EXPERIMENTAL DERMATOLOGY, WILEY-BLACKWELL, Marburg, GERMANY, pp. e43 - e43, presented at 39th Annual Meeting of the Arbeitsgemeinschaft-Dermatologische-Forschung (ADF), Marburg, GERMANY, 01 - 03 March 2012 (link is external)
Conference Posters
Hardeman EC; Kee AJ; Lucas CA; Chagan J; Gunning PW, 2016, 'Metabolic impact of targeting the tropomyosin/actin cytoskeleton to treat cancer', in MOLECULAR BIOLOGY OF THE CELL, AMER SOC CELL BIOLOGY, San Francisco, CA, Vol. 27, presented at Annual Meeting of the American-Society-for-Cell-Biology (ASCB), San Francisco, CA, 03 - 07 December 2016 (link is external)
Suchowerska A; Chen B; Mueller J; Ittner A; Hardeman E; Ittner L; Janitz M; Gunning P; Fath T, 2013, 'Increased levels of actin filaments containing the tropomyosin Tm5NM1 regulate excitotoxicity in the mouse', in MOLECULAR BIOLOGY OF THE CELL, AMER SOC CELL BIOLOGY, Vol. 24 (link is external)
Conference Presentations
Jalilian I; Schevzov G; Fath T; Heu C; Bischof L; Vindin H; Stehn JR; Tate MK; Hardeman EC; Gunning PW, 2014, 'Determining the impact of the actin cytoskeleton on the mechanical properties of cells', in MOLECULAR BIOLOGY OF THE CELL, AMER SOC CELL BIOLOGY, Philadelphia, PA, Vol. 25, presented at ASCB/IFCB Meeting, Philadelphia, PA, 06 - 10 December 2014 (link is external)
Hardeman EC; Yang L; Kee AJ; Polishchuk E; Polishchuk R; Pleines I; Kile B; Weigert R; Gunning PW, 2014, 'A novel actin filament population defined by the tropomyosin Tm4 regulates ER-to-Golgi trafficking.', in MOLECULAR BIOLOGY OF THE CELL, AMER SOC CELL BIOLOGY, Philadelphia, PA, Vol. 25, presented at ASCB/IFCB Meeting, Philadelphia, PA, 06 - 10 December 2014 (link is external)
Brayford S; Schevzov G; Hardeman EC; Bear JE; Gunning PW, 2014, 'Tropomyosin 5a/b actin filaments collaborate with arp2/3 actin filaments to regulate cell motility.', in MOLECULAR BIOLOGY OF THE CELL, AMER SOC CELL BIOLOGY, Philadelphia, PA, Vol. 25, presented at ASCB/IFCB Meeting, Philadelphia, PA, 06 - 10 December 2014 (link is external)
Schevzov G; Wang B; Hook JW; Tonthat C; Pleines I; Kile B; Hardeman EC; Gunning PW, 2014, 'Distinct Actin Filament Networks Modulate Different Cell Signalling Pathways Involved in Cell Proliferation and Senescence.', in MOLECULAR BIOLOGY OF THE CELL, AMER SOC CELL BIOLOGY, Philadelphia, PA, Vol. 25, presented at ASCB/IFCB Meeting, Philadelphia, PA, 06 - 10 December 2014 (link is external)
Jalilian I; Schevzov G; Fath T; Heu C; Bischof L; Vindin H; Stehn JR; Tate MK; Hardeman EC; Gunning PW, 2014, 'Determining the impact of the actin cytoskeleton on the mechanical properties of cells.', in MOLECULAR BIOLOGY OF THE CELL, AMER SOC CELL BIOLOGY, Philadelphia, PA, Vol. 25, presented at ASCB/IFCB Meeting, Philadelphia, PA, 06 - 10 December 2014 (link is external)
Conference Abstracts
Eslami S; Boysen L; Amorim N; Bould S; Kee A; Lucas C; Johnston K; Hardeman E; Cohn RJ; Barres R, 2015, 'Radiation-induced metabolic complications: potential role for the microbiota', in Radiation-induced metabolic complications: potential role for the microbiota, Nutrient & Metabolite Sensing, Copenhagen, Denmark, presented at Nutrient & Metabolite Sensing, Copenhagen, Denmark, 01 - 05 November 2015
Eslami S; Boysen L; Amorim N; Bould S; Kee A; Lucas C; Johnston K; Cohn R; Hardeman E; Barres R, (eds.), 2015, 'Metabolic complications in cancer survivors: role of adipose tissue dysfunction', in Metabolic complications in cancer survivors: role of adipose tissue dysfunction, Keystone Symposia: Systems Biology of Lipid Metabolism, Breckenridge, Colorado, USA, presented at Keystone Symposia: Systems Biology of Lipid Metabolism, Breckenridge, Colorado, USA, 09 - 13 February 2015
Segueira VB; Stehn JR; Haass N; Bonello T; Guven K; Desouza M; Schevzov G; Kottyan G; McCluskey A; Bischof L, 2012, 'Improving the specificity of drugs which target the actin cytoskeleton for cancer therapy', in CANCER RESEARCH, AMER ASSOC CANCER RESEARCH, Vol. 72 (link is external)